CST, 3396T, ZEB1 (D80D3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZEB1/TCF8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZEB1 (D80D3) Rabbit Monoclonal Antibody (CST #3396) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZEB1 (D80D3) Rabbit Monoclonal Antibody detects endogenous levels of total TCF8/ZEB1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region around residue Asp868 of human TCF8/ZEB1. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8). Alternate Names AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; MGC133261; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF-8; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG