CST, 34050T, PI3 Kinase p110 delta (D1Q7R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PI3K p110 delta. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PI3 Kinase p110 delta (D1Q7R) Rabbit Monoclonal Antibody (CST #34050) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PI3 Kinase p110 delta (D1Q7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PI3 Kinase Class III δ protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His481 of human PI3 Kinase Class III δ protein. Background Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Alternate Names APDS; IMD14; p110D; P110DELTA; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 20; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide; phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta; PI3-kinase p110 subunit delta; PI3-kinase subunit delta; PI3K; PI3K-delta; PI3Kdelta; PIK3CD; PK3CD; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG