CST, 34097P, YAP/TAZ (D24E4) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying TAZ/YAP (YAP1-2gamma). Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, YAP/TAZ (D24E4) Rabbit Monoclonal Antibody (Biotinylated) (CST #34097) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity YAP/TAZ (D24E4) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total YAP and TAZ proteins. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp362 of human TAZ protein. Background YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size (6-8). Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteasomal degradation of YAP (10). Alternate Names 65 kDa Yes-associated protein; COB1; DKFZp586I1419; FLJ27004; FLJ45718; Protein yorkie homolog; TAZ; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; Transcriptional coactivator YAP1; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; WWTR1; YAP; YAP1; YAP2; YAP65; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; Yes1 associated transcriptional regulator; YKI; Yorkie homolog Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 55, 78 Source/Isotype: Rabbit IgG