CST, 34141S, LAMP2 (E6A6S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP2 mouse. Validated for WB,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP2 (E6A6S) Rabbit Monoclonal Antibody (CST #34141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:1600 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LAMP2 (E6A6S) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP2 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse LAMP2 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names CD107 antigen-like family member B; CD107b; Lam; Lamp; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; lysosome-associated membrane protein-2; Mac; Mac3; OTTMUSP00000018739; OTTMUSP00000018801 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 100-130 Source/Isotype: Rabbit IgG