CST, 34149SF, CD141/Thrombomodulin (E7Y9P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD141. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD141/Thrombomodulin (E7Y9P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #34149) is ready to ship. Product Usage Information This product is the carrier free version of product #43514. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 43514 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD141/Thrombomodulin (E7Y9P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD141/Thrombomodulin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD141/Thrombomodulin recombinant protein. Background CD141/Thrombomodulin (TM, THBD, BDCA-3) is an integral membrane protein expressed on the surface of endothelial cells (1). Acting as a cofactor with Thrombin, CD141/Thrombomodulin activates and initiates the Protein C anticoagulant pathway (1,2). CD141/Thrombomodulin is expressed by a small subset of human CD11c+ myeloid dendritic cells (3-4). These CD141+XCR1+ dendritic cells cross-present antigens to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells (3-5). Alternate Names AHUS6; BDCA3; CD141; CD141 antigen; Fetomodulin; THBD; THPH12; THRM; Thrombomodulin; TM; TRBM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG