CST, 3419S, Streptavidin (Sepharose® Bead Conjugate)

Affinity and Purification Reagents for studying in the research area. Product Usage Information Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details. Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4). Source / Purification Streptavidin is expressed in . Background Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2). Alternate Names Bead Conjugate; Biotinylated; Streptavidin