CST, 34209SF, CEACAM7 (E5Q6X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CEACAM7. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CEACAM7 (E5Q6X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #34209) is ready to ship. Product Usage Information This product is the carrier free version of product #81945. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81945 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CEACAM7 (E5Q6X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CEACAM7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val141 of human CEACAM7 protein. Background Carcinoembryonic antigen (CEA)-related cell adhesion molecule 7 (CEACAM7) is a homodomeric glycoprotein that is covalently linked to the plasma membrane via a GPI anchor and modulates cell adhesion via its N-terminal IgV domain (1). In normal human tissues, CEACAM7 expression is largely restricted to epithelial cells of the colon and pancreatic ducts (2). While some research studies have demonstrated that CEACAM7 expression is downregulated in human colorectal carcinomas, other studies have highlighted high expression levels in some pancreatic ductal adenocarcinomas (PDAC) (3-5). Given the restricted expression pattern in normal human tissues, CEACAM7 is positioned as an attractive immunotherapeutic target for PDAC (4). Alternate Names Carcinoembryonic antigen CGM2; carcinoembryonic antigen gene family member 2; carcinoembryonic antigen related cell adhesion molecule 7; Carcinoembryonic antigen-related cell adhesion molecule 7; CEA; CEA cell adhesion molecule 7; CEACAM7; CEAM7; CGM2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG