CST, 34265S, Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody

Monoclonal Antibody for studying FOLR1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody (CST #34265) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 30124 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Folate Receptor Alpha/FOLR1 (E8U2F) Mouse Monoclonal Antibody recognizes endogenous levels of total Folate Receptor Alpha/FOLR1 protein. Non-specific staining was observed in normal human brain by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp77 of human Folate Receptor Alpha/FOLR1 protein. Background Folate Receptor Alpha (FOLR1) is a cysteine-rich cell-surface receptor that plays a critical role in the maintenance and transport of folate (vitamin B9) and reduced folic acid derivatives into cells and is essential for various cellular processes (1,2). FOLR1 binds to folate and facilitates its internalization into cells through receptor-mediated endocytosis or potocytosis and is important in cellular processes, including DNA synthesis and repair (1-3). Intracellular folate levels are critical to maintaining and supporting folate functions in its multiple coenzyme forms (4). It is primarily expressed in tissues requiring high folate levels, such as the placenta, kidneys, and certain cancer cells (5). FOLR1 has been found to be overexpressed in several types of cancer, including ovarian, lung, and breast cancer (6-8). Alternate Names Adult folate-binding protein; FBP; folate binding protein; Folate receptor 1; folate receptor 1 (adult); Folate receptor alpha; Folate receptor, adult; FOLR; FOLR1; FR-alpha; FRalpha; KB cells FBP; Ovarian tumor-associated antigen MOv18 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Mouse IgG1 kappa