CST, 3429S, Phospho-NuMA (Ser395) Antibody

Polyclonal Antibody for studying NuMA-1 (Ser395) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-NuMA (Ser395) Antibody (CST #3429) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-NuMA (Ser395) Antibody detects endogenous levels of NuMA protein only when phosphorylated at Ser395. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser395 of human NuMA. Antibodies are purified using protein A and peptide affinity chromatography. Background The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4). Phosphorylation at Thr2055 by CDK1 is required for spindle pole association of NuMA at the onset of mitosis. Dephosphorylation by PPP2CA leads to enhancement of NuMA at the cell cortex in anaphase and proper cell-cycle progression (5,6). Phospho-NuMA (Ser395) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser395 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus , CST's modification site knowledgebase, at www.phosphosite.org for more information. Alternate Names centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; NMP22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NUMA; NuMA protein; NuMA-1; NUMA1; SP-H antigen; structural nuclear protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 238 SOURCE: Rabbit