CST, 34300S, Zap-70 (D1C10E) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3113)

Monoclonal Antibody for studying ZAP70. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Zap-70 (D1C10E) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3113) (CST #34300) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Zap-70 (D1C10E) XP Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human Zap-70. Background The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Alternate Names 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; FLJ17670; FLJ17679; IMD48; SRK; STCD; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG