CST, 3430T, RhoC (D40E4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RhoC. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RhoC (D40E4) Rabbit Monoclonal Antibody (CST #3430) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RhoC (D40E4) Rabbit Monoclonal Antibody detects endogenous levels of total RhoC protein. This antibody does not cross-react with RhoA or RhoB. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human RhoC. Background Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3). Alternate Names ARH9; ARHC; epididymis secretory sperm binding protein; h9; MGC1448; MGC61427; oncogene RHO H9; ras homolog family member C; ras homolog gene family, member C; RAS-related homolog 9; Rho cDNA clone 9; Rho-related GTP-binding protein RhoC; RHOC; rhoC GTPase; RHOH9; small GTP binding protein RhoC Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG