CST, 3431S, Id2 (D39E8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ID2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Id2 (D39E8) Rabbit Monoclonal Antibody (CST #3431) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Id2 (D39E8) Rabbit Monoclonal Antibody detects endogenous levels of total Id2 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn97 of human Id2 protein. Background Inhibitor of DNA-binding-2 (Id2) is a member of the Id proteins which belong to the helix-loop-helix (HLH) protein family. The Id protein functions by binding to specific transcription factors and preventing their dimerization and DNA binding (1-3). Id2 interacts with a wide variety of transcription factors including E proteins (5), TCS (4), Pax (3) and the tumor suppressor Rb (1). Id2 has been shown to be important in regulating cellular differentiation, proliferation, development and tumorgenesis (6-8). In tumor cells, increased levels of Id2 functionally inactivate Rb, leading to cellular transformation and cancer (9,10). Id2 is therefore a promising therapeutic target for treatment of cancer (11). Alternate Names BHLHB26; cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; GIG8; helix-loop-helix protein ID2; ID2; ID2A; ID2H; inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; inhibitor of DNA binding 2, HLH protein; MGC26389 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG