CST, 3433S, RECK (D8C7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RECK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RECK (D8C7) Rabbit Monoclonal Antibody (CST #3433) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RECK (D8C7) Rabbit Monoclonal Antibody detects endogenous levels of total RECK protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile913 of human RECK protein. Background RECK (reversion-inducing cysteine-rich protein with Kazal motif) is a GPI-anchored membrane glycoprotein that negatively regulates members of the matrix metalloproteinase (MMP) family and functions as a suppressor of transformation (1,2). Its function in MMP inhibition makes RECK a crucial factor in the regulation of extracellular matrix formation and stability during development (2-4). RECK has also been linked to the regulation of other extracellar matrix proteases such as ADAM10 and CD13 and functions in modulating target protein endocytosis and Notch signaling (5,6). RECK is widely expressed in normal tissue and decreased expression of RECK due to promoter methylation has been correlated with tumor transformation, angiogenesis and metastasis (1,7-9). Therefore, loss of RECK expression serves as a prognostic hallmark for cancer malignancy (10,11) Alternate Names hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion inducing cysteine rich protein with kazal motifs; Reversion-inducing cysteine-rich protein with Kazal motifs; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs); Suppressor of tumorigenicity 15 protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG