CST, 3436S, Flotillin-2 (C42A3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying flotillin-2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Flotillin-2 (C42A3) Rabbit Monoclonal Antibody (CST #3436) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Flotillin-2 (C42A3) Rabbit Monoclonal Antibody detects endogenous levels of total flotillin-2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe157 of human flotillin-2 protein. Background Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located in noncaveolar microdomains (lipid rafts) on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, and the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. Alternate Names ECS-1; ECS1; Epidermal surface antigen; ESA; ESA1; FLOT2; flotillin 2; Flotillin 2 (epidermal surface antigen 1); Flotillin-2; M17S1; Membrane component chromosome 17 surface marker 1; membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49, 58 Source/Isotype: Rabbit IgG