CST, 3437S, AMPA Receptor 3 (GluA 3) Antibody

Polyclonal Antibody for studying GluR3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, AMPA Receptor 3 (GluA 3) Antibody (CST #3437) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity AMPA Receptor 3 (GluA 3) Antibody detects endogenous levels of total GluA 3 protein. This antibody is not predicted to detect other AMPA receptors subunits (e.g. GluA 1, GluA 2 or GluA 4) based on sequence homology of the antigen. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro590 of human GluA 3. Antibodies are purified by protein A and peptide affinity chromatography. Background AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4) that assemble as homo- or hetero-tetramers and mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization and plasticity. Post-transcriptional modifications (alternative splicing and nuclear RNA editing) and post-translational modifications (glycosylation, phoshorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs (1). GluR 3 knockout mice exhibited normal basal synaptic transmission and long-term depression (LTD) but enhanced long-term potentiation (LTP). In contrast, GluR 2/3 double knockout mice are impaired in basal synaptic transmission (2). Aberrant GluR 3 expression or activity is implicated in a number of diseases, including autoimmune epilepsy, X-linked mental retardation, Rett's syndrome, amyotrophic lateral sclerosis and Alzheimer disease (3). Alternate Names AMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GluR-3; GluR-C; GluR-K3; GLUR3; GLURC; glutamate ionotropic receptor AMPA type subunit 3; Glutamate receptor 3; glutamate receptor C; Glutamate receptor ionotropic, AMPA 3; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; GRIA3; MRX94; MRXSW Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit