CST, 34418T, PrimPol (F5E9I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PrimPol. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PrimPol (F5E9I) Rabbit Monoclonal Antibody (CST #34418) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PrimPol (F5E9I) Rabbit Monoclonal Antibody recognizes endogenous levels of total PrimPol protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human PrimPol protein. Background DNA replication stress, a major source of genome instability, results from barriers to DNA replication fork progression, including DNA damage lesions, transcription-replication conflicts, and nucleotide depletion. Multiple pathways are in place to stabilize replication forks under stress, and to promote DNA replication restarting (1). PrimPol is a nuclear and mitochondrial primase and DNA-directed polymerase belonging to the archaea-eukaryotic primases (AEP) superfamily. PrimPol catalyzes prepriming of DNA after replication stalling, allowing DNA replication to continue in the presence of DNA damage. The repriming occurs beyond the DNA lesion, leaving small single-stranded DNA (ssDNA) gaps that are subsequently repaired (2,3). PrimPol abundance is regulated by ubiquitin-mediated proteasomal degradation, and deubiquitination by USP36 stabilizes PrimPol in response to replication stress (4). PrimPol activity is negatively regulated through phosphorylation by PLK1 at Ser538 (5), and positively regulated through ATR/Chk1-dependent phosphorylation at Ser255 (6). Aberrant expression of PrimPol has been observed in human cancers, and its expression in cancer may confer vulnerability to therapies targeting DNA repair pathways (2,7). Alternate Names CC111; CCDC111; coiled-coil domain containing 111; Coiled-coil domain-containing protein 111; DNA-directed primase/polymerase protein; FLJ33167; hPrimpol1; MYP22; primase and DNA directed polymerase; PRIMPOL; Primpol1; PRIPO Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG