CST, 34423T, GOT1 (E4A4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GOT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GOT1 (E4A4O) Rabbit Monoclonal Antibody (CST #34423) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GOT1 (E4A4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total GOT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala344 of human GOT1 protein. Background Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the interconversion of aspartate and oxaloacetate (1). The increased transamination primarily catalyzed by GOT1 leads to elevated levels of 2-hydroxyglutarate, which promotes methylation of the gene locus, inhibits Foxp3 expression and activates T helper 17 (T 17) cell differentiation (2). In addition, GOT1 is critical to the survival of cells with electron transport chain inhibition by generating aspartate, a metabolite determining the proliferation of these cells (3-4). Studies also show that GOT1 plays a key role in the noncanonical glutamine pathway that supports liver tumorigenesis (5). Alternate Names AATC; aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; GIG18; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); GOT1; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG