CST, 34425T, S100A9 (D5O6O) Rabbit Monoclonal Antibody (IHC Formulated)

Monoclonal Antibody for studying S100A9. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, S100A9 (D5O6O) Rabbit Monoclonal Antibody (IHC Formulated) (CST #34425) is ready to ship. Product Usage Information IHC Leica Bond: 1:800 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity S100A9 (D5O6O) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total S100A9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human S100A9 protein. Background S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9). Alternate Names 60B8AG; CAGB; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; L1AG; Leukocyte L1 complex heavy chain; LIAG; MAC387; MIF; Migration inhibitory factor-related protein 14; MRP-14; MRP14; NIF; p14; Protein S100-A9; S100 calcium binding protein A9; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A9; S10A9 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG