CST, 3444S, HA-Tag (6E2) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, HA-Tag (6E2) Mouse Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #3444) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HA-Tag (6E2) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). The antibody was conjugated to Alexa Fluor 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1