CST, 3445S, GST-Tag (26H1) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, GST-Tag (26H1) Mouse Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #3445) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GST-Tag (26H1) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a GST fusion protein. This antibody was conjugated to Alexa Fluor 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2a