CST, 34464SF, CD109 (E4I2V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD109. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD109 (E4I2V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #34464) is ready to ship. Product Usage Information This product is the carrier free version of product #24765. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 24765 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD109 (E4I2V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD109 protein. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val834 of human CD109 protein. Background CD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins (1). CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling (2,3). Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling (4-7). CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors (8-10). Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers (11-14). Alternate Names 150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109; CD109 antigen; CD109 molecule; CPAMD7; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; r150; RP11-525G3.1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG