CST, 3447T, Notch1 (5B5) Rat Monoclonal Antibody

Monoclonal Antibody for studying Notch1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Notch1 (5B5) Rat Monoclonal Antibody (CST #3447) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31289 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Notch1 (5B5) Rat Monoclonal Antibody detects endogenous levels of total Notch1 protein. This antibody recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). This antibody antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases. Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Alternate Names AOS5; AOVD1; hN1; Neurogenic locus notch homolog protein 1; NEXT; NICD; NOTC1; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch homolog 1, translocation-associated; Notch homolog 1, translocation-associated (Drosophila); notch receptor 1; NOTCH1; TAN1; Translocation-associated notch protein TAN-1 Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 120, 300 Source/Isotype: Rat IgG2b