CST, 3448S, TDP43 (G400) Antibody

Polyclonal Antibody for studying TDP43. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, TDP43 (G400) Antibody (CST #3448) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TDP43 (G400) Antibody detects endogenous levels of total TDP43 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly400 of human TDP43. Antibodies are purified by protein A and peptide affinity chromatography. Background TDP43 (TAR DNA-binding protein 43) is involved in transcriptional regulation and exon splicing (1,2). While normal TDP43 is a nuclear protein, pathological TDP43 is a component of insoluble aggregates in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). In these disorders, TDP43 is abnormally ubiquitinated, phosphorylated, and cleaved to generate carboxy-terminal fragments that are sequestered as insoluble aggregates in neuronal nuclei, perikarya, and neurites (3,4). Additionally, TDP43 inhibits the expression of the gene and regulates gene splicing (1,5). Alternate Names ALS10; TADBP; TAR DNA binding protein; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TARDBP; TDP-43; TDP43 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit