CST, 3451S, Phospho-mu-Opioid Receptor (Ser375) Antibody

Polyclonal Antibody for studying MOR-1 (Ser377) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-mu-Opioid Receptor (Ser375) Antibody (CST #3451) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-mu-Opioid Receptor (Ser375) Antibody detects transfected µ-opioid receptor only when phosphorylated at Ser375 of mouse MOR (or Ser377 of human MOR). Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human (homologous to Ser375 of mouse) μ-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography. Background The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides (1). Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase (1) and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades (2,3). Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance (4,5). As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains (6). Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization (7). Alternate Names hMOP; KIAA0403; LMOR; M-OR-1; MOP; MOR; MOR-1; MOR1; Mu opiate receptor; Mu opioid receptor; mu opioid receptor hMOR-1a; Mu-type opioid receptor; opioid receptor mu 1; opioid receptor, mu 1; OPRM; OPRM1 Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 70 to 90 SOURCE: Rabbit