CST, 34584S, FoxK1 (F8W6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXK1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxK1 (F8W6Q) Rabbit Monoclonal Antibody (CST #34584) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FoxK1 (F8W6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a central region of human FoxK1 protein. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4). FoxK1 belongs to a subfamily of Fox proteins along with FoxK2. FoxK1 functions as a transcriptional repressor of downstream target genes, including , , , , and (5-7). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. The β isoform has a truncated C terminus compared to the α isoform. The two isoforms of FoxK1 show reciprocal expression patterns during muscle regeneration. FoxK1-β is expressed mainly in quiescent satellite cells (8), whereas FoxK1-α is the dominant isoform in proliferating myoblasts from activated satellite cells (9). Both isoforms of FoxK1 jointly regulate proliferation and differentiation of myogenic stem cells during development and muscle regeneration (10). Alternate Names FLJ14977; forkhead box K1; Forkhead box protein K1; FOXK1; FOXK1L; MNF; Myocyte nuclear factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG