CST, 3462S, FLT3 (8F2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FLT3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FLT3 (8F2) Rabbit Monoclonal Antibody (CST #3462) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 43128 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FLT3 (8F2) Rabbit Monoclonal Antibody detects endogenous levels of FLT3 protein. This antibody does not cross-react with related proteins. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser740 of human FLT3. Background FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). Alternate Names CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK-2; FLK2; FLT-3; FLT3; FLT3 receptor tyrosine kinase; fms related receptor tyrosine kinase 3; fms related tyrosine kinase 3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Receptor-type tyrosine-protein kinase FLT3; Stem cell tyrosine kinase 1; STK-1; STK1; Tyrosine-protein kinase receptor FLT3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 nonglycosylated form;160 glycosylated mature form Source/Isotype: Rabbit IgG