CST, 34639S, ROR alpha (E6G5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RORalpha1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ROR alpha (E6G5I) Rabbit Monoclonal Antibody (CST #34639) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ROR alpha (E6G5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total RORα protein. This antibody does not cross-react with other ROR proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human RORα protein. Background Retinoic acid-receptor (RAR)-related orphan receptor alpha (RORα) is an orphan nuclear receptor that, along with RORβ and RORγ, can bind to DNA as monomers. The N-terminus is alternatively spliced, resulting in isoforms that have various DNA binding properties (1). Activation of target genes by RORα is opposed by Rev-erb and NCoR (2). RORα has been implicated in many distinct biological functions. RORα regulates networks during Purkinje cell development, and its deletion causes the staggerer phenotype in mouse models (3). These mice display thin, long bones versus wild-type counterparts, as RORα is expressed in mesenchymal stem cells from bone marrow (4). RORα also upregulates IκBα, resulting in the inhibition of Nf-κB p65 and the inflammation response (5). RORα and RORγ facilitate the differentiation of T helper 17 cells, which mediate tissue inflammation (6). Also, RORα can promote myoblast differentiation by interacting with p300 and MyoD (7). Additionally, RORα plays a role in circadian rhythm by controlling key genes such as (8). Alternate Names DKFZp686M2414; IDDECA; MGC119326; MGC119329; NR1F1; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR related orphan receptor A; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR-alpha; ROR1; ROR2; ROR3; RORA; RORA iso1; RZR-ALPHA; RZRA; thyroid hormone nuclear receptor alpha variant 4; transcription factor RZR-alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG