CST, 3463S, Phospho-FLT3 (Tyr969) (C24D9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FLT3 (Tyr969) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FLT3 (Tyr969) (C24D9) Rabbit Monoclonal Antibody (CST #3463) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-FLT3 (Tyr969) (C24D9) Rabbit Monoclonal Antibody detects endogenous levels of FLT3 only when phosphorylated at Tyr969. This antibody may cross-react with other tyrosine-phosphorylated proteins. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Tyr969 of human FLT3. Background FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb is directed against a previously unpublished FLT3 tyrosine phosphorylation site at Tyr969 that was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of FLT3 at Tyr969 was observed in various leukemia, lymphoma and carcinoma cell lines and in tumors. Alternate Names CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK-2; FLK2; FLT-3; FLT3; FLT3 receptor tyrosine kinase; fms related receptor tyrosine kinase 3; fms related tyrosine kinase 3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Receptor-type tyrosine-protein kinase FLT3; Stem cell tyrosine kinase 1; STK-1; STK1; Tyrosine-protein kinase receptor FLT3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG