CST, 34642S, Clusterin (D7N2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Clusterin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Clusterin (D7N2K) Rabbit Monoclonal Antibody (CST #34642) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83411 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Clusterin (D7N2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Clusterin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser396 of human Clusterin protein. Background Clusterin (CLU, apolipoprotein J) is a multifunctional glycoprotein that is expressed ubiquitously in most tissues. Clusterin functions as a secreted chaperone protein that interacts with and stabilizes stress-induced proteins to prevent their precipitation (1,2). Research studies show that clusterin plays a protective role in Alzheimer's disease by sequestering amyloid β(1-40) peptides to form long-lived, stable complexes, which prevents amyloid fibril formation (3-5). In addition to the secreted protein, several intracellular isoforms are localized to the nucleus, mitochondria, cytoplasm, and ER. The subcellular distribution of these multiple isoforms leads to the diversity of clusterin functions. Additional studies report that clusterin is involved in membrane recycling, cell adhesion, cell proliferation, apoptosis, and tumor survival (6-9). The clusterin precursor is post-translationally cleaved into the mature clusterin α and clusterin β forms. Clusterin α and β chains create a heterodimer through formation of disulfide bonds (10). Alternate Names AAG4; Aging-associated gene 4 protein; aging-associated protein 4; Apo-J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; CLU1; CLU2; CLUS; Clusterin; Clusterin alpha chain; Clusterin beta chain; Complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; Complement-associated protein SP-40,40; epididymis secretory sperm binding protein; Ku70-binding protein 1; KUB1; MGC24903; NA1/NA2; SGP-2; SGP2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; TRPM-2; TRPM2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-42, 65, 75 Source/Isotype: Rabbit IgG