CST, 34661S, E2F8 (E8I3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying E2F8. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, E2F8 (E8I3L) Rabbit Monoclonal Antibody (CST #34661) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity E2F8 (E8I3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total E2F8 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human E2F8 protein. Background The E2F family consists of eight transcription factors that regulate gene expression to control cell cycle progression. The atypical E2F family members, E2F-7 and E2F-8, are unique in that they have two distinct DNA-binding domains, but lack the DP-heterodimerization, retinoblastoma-binding, and transcriptional activation domains contained in the canonical E2F family members. E2F-7 and E2F-8 are thought to function primarily as transcriptional repressors (1). E2F8 is highly expressed in multiple human cancers, including hepatocellular carcinoma, lung, breast, ovarian and cervical cancer, and may serve as a prognostic marker (1-5). In cancer cell lines, E2F8 expression enhances proliferation, migration, invasion, and the expression of epithelial mesenchymal transition (EMT) markers (2,3). Alternate Names E2F family member 8; E2F transcription factor 8; E2F-8; E2F8; FLJ23311; Transcription factor E2F8 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG