CST, 3469S, eIF4H (D85F2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EIF4H. Validated for WB,IP,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF4H (D85F2) Rabbit Monoclonal Antibody (CST #3469) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity eIF4H (D85F2) Rabbit Monoclonal Antibody detects endogenous levels of total eIF4H protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF4H. Background A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5). Alternate Names eIF-4H; EIF4H; Eukaryotic translation initiation factor 4H; IF4H; KIAA0038; WBSCR1; Williams-Beuren syndrome chromosomal region 1 protein; Williams-Beuren syndrome chromosome region 1; WSCR1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25, 27 Source/Isotype: Rabbit IgG