CST, 34735S, EXOSC10 (F4Q2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EXOSC10. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, EXOSC10 (F4Q2B) Rabbit Monoclonal Antibody (CST #34735) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EXOSC10 (F4Q2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total EXOSC10 protein. This antibody detects a 32 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly877 of human EXOSC10 protein. Background Exosome component 10 (EXOSC10), also known as RRP6 or autoantigen PM/Scl-100, is a 3'-5' nuclear component of the exosome complex with 3'-5' exonuclease activity. EXOSC10 is essential for the 3' end formation of 5.8S rRNA, as well as the degradation of improperly spliced or polyadenylated mRNAs (1-3). Additionally, EXOSC10 regulates the proper 3' formation of small nucleolar RNAs (snoRNAs) (4). EXOSC10 is the antibody target in polymyositis-scleroderma overlap, an autoimmune disease (5-7). Alternate Names autoantigen PM-SCL; Autoantigen PM/Scl 2; EXOSC10; Exosome complex component 10; Exosome component 10; EXOSX; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL; PMSCL2; Polymyositis/scleroderma autoantigen 100 kDa; Polymyositis/scleroderma autoantigen 2; polymyositis/scleroderma autoantigen 2 (100kD); polymyositis/scleroderma autoantigen 2, 100kDa; RRP6; Rrp6p Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG