CST, 3476S, Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying FGFR1 (Tyr653/Tyr654) phosphate. Validated for Western Blotting,Simple Western™. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse Monoclonal Antibody (CST #3476) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse Monoclonal Antibody detects endogenous levels of FGF receptors only when phosphorylated at tyrosines 653/654. This antibody detects phosphorylated FGF Receptors 2 and 4 when expressed exogenously. Based on sequence comparisons, reactivity with FGF Receptor 3 is possible but has not been experimentally confirmed. This antibody also cross-reacts slightly with activated PDGF and insulin/IGF-I receptors. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1. The corresponding sequence is identical in FGF receptor-2, -3 and -4. Background Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). Alternate Names Basic fibroblast growth factor receptor 1; bFGF-R-1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1; FGFR1/PLAG1 fusion; Fibroblast growth factor receptor 1; FLG; FLJ99988; FLT-2; FLT2; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 120, 145 Source/Isotype: Mouse IgG2b