CST, 3480S, Pyk2 (5E2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Pyk2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pyk2 (5E2) Mouse Monoclonal Antibody (CST #3480) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pyk2 (5E2) Mouse Monoclonal Antibody detects endogenous levels of total Pyk2 protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2. Background Protein-tyrosine kinase 2 (Pyk2), also called CAKβ, RAFTK, and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7). Alternate Names CADTK; CAK beta; CAK-beta; CAKB; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2; FAK2; Focal adhesion kinase 2; PKB; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; Related adhesion focal tyrosine kinase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 116 Source/Isotype: Mouse IgG2a