CST, 3481S, Phospho-Ephrin B (Tyr324/329) Antibody

Polyclonal Antibody for studying Ephrin B1 (Tyr324/Tyr329) phosphate/Ephrin B3 (Tyr318/Tyr323) phosphate/Ephrin B2 (Tyr311/Tyr316) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Ephrin B (Tyr324/329) Antibody (CST #3481) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Ephrin B (Tyr324/329) Antibody detects transfected levels of ephrin B protein only when phosphorylated at tyrosines 324/329. This antibody cross-reacts with ephrins B1, B2 and B3 but does not cross-react with other tyrosine-phosphorylated proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr324/329 of human ephrin B1. Antibodies are purified by protein A and peptide affinity chromatography. Background The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 (7). Alternate Names CFND; CFNS; EFB1; EFL-3; EFL3; EFL6; EFNB1; EFNB2; EFNB3; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; Ephrin B1; ephrin B2; Ephrin B3; Ephrin-B1; Ephrin-B1 C-terminal fragment; Ephrin-B1 CTF; Ephrin-B1 ICD; Ephrin-B1 intracellular domain; Ephrin-B2; Ephrin-B3; EPLG2; EPLG5; EPLG8; HTK ligand; HTK-L; HTKL; LERK-2; LERK-5; LERK-8; LERK2; LERK5; LERK8; ligand of eph-related kinase 2; ligand of eph-related kinase 5; MGC126226; MGC126227; MGC126228; MGC8782 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 59 SOURCE: Rabbit