CST, 34822T, TIM23 (E1Q7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIMM23. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TIM23 (E1Q7L) Rabbit Monoclonal Antibody (CST #34822) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TIM23 (E1Q7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIM23 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala29 of human TIM23 protein. Background TIM23 is a subunit of the mitochondrial import inner membrane translocase complex. This complex mediates translocation of presequence-containing proteins into the mitochondrial matrix and inner membrane. Besides TIM23, there are multiple subunits in the complex, including TIM17A, TIM17B, and TIM50 (1,2). Studies show that mutant huntingtin (mHTT), the protein causing neurodegenerative Huntington's disease, binds to TIM23 subunit. There are less soluble matrix mitochondrial proteins imported by the TIM23 complex in cells expressing mHTT and in the brain tissues of Huntington's disease patients when compared with controls (2). In addition, asbestos causes translocation of NOX4 to the mitochondrial matrix through direct interaction with TIM23 in lung macrophages. TIM23 increases NOX4-induced reactive oxygen species (ROS) in mitochondria and metabolic reprogramming to oxidative phosphorylation (3). Alternate Names LOC10431; MGC22767; Mitochondrial import inner membrane translocase subunit Tim23; TIM23; TIMM23; translocase of inner mitochondrial membrane 23; translocase of inner mitochondrial membrane 23 homolog; translocase of inner mitochondrial membrane 23 homolog (yeast)-like; translocase of the inner mitochondrial membrane Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG