CST, 3482T, Phospho-LKB1 (Ser428) (C67A3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LKB1 (Ser428) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-LKB1 (Ser428) (C67A3) Rabbit Monoclonal Antibody (CST #3482) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-LKB1 (Ser428) (C67A3) Rabbit Monoclonal Antibody detects endogenous levels of LKB1 when phosphorylated at Ser428. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser428 of human LKB1 (Ser431 of mouse). Background LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7). Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 (8) as well as regulate apoptosis through PTEN signaling pathway suppression of Akt (9). Alternate Names hLKB1; Liver kinase B1; LKB1; PAR-4; PAR4; PJS; polarization-related protein LKB1; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Serine/threonine-protein kinase STK11; STK11 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit