CST, 34852S, NYAP1 Antibody

Polyclonal Antibody for studying NYAP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, NYAP1 Antibody (CST #34852) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NYAP1 Antibody recognizes endogenous levels of total NYAP1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys361 of human NYAP1 protein. Antibodies are purified by peptide affinity chromatography. Background Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 1 (NYAP1) is a member of the Nyap family of phosphoproteins, including NYAP1, NYAP2, and Myosin16/NYAP3. Upon stimulation by contactin5, NYAPs are phosphorylated by Fyn. Once phosphorylated, NYAP1, as well as other members of this family, interacts with PI3K p85 and active PI3K, Akt, and Rac1. The interaction of NYAP1 with WAVE complex mediates remodeling of the actin cytoskeleton after activation by PI3K-produced PIP3 and Rac1 (1). The phosphorylation of NYAP1 directly contributes to normal neuronal migration and morphogenesis of neurons, acting downstream of Fyn, which is a non-receptor protein-tyrosine kinase that is a member of the Src kinase family (2). The overexpression of NYAP1 promotes neurite branching and elongation at early neuronal migration compared to the NYAP1-KO model. The lack of NYAP1 induces thinner leading neurites affecting the multibipolar morphology of neurons (2). Alternate Names C7orf51; CG051; chromosome 7 open reading frame 51; FLJ37538; neuronal tyrosine phosphorylated phosphoinositide-3-kinase adaptor 1; Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 1; neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 1; NYAP1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 105 SOURCE: Rabbit