CST, 34858S, SLFN11 (D8W1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLFN11. Validated for WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, SLFN11 (D8W1B) Rabbit Monoclonal Antibody (CST #34858) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24571 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SLFN11 (D8W1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLFN11 protein. Non-specific staining was observed in skeletal muscle. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SLFN11 protein. Background SLFN11 is a nuclear protein that belongs to the Schlafen (SLFN) family of genes involved in cell cycle regulation and growth inhibition (1,2). Expression of SLFN11 predicts sensitivity of cancer cell lines to DNA-damaging agents (1,3). Evidence suggests that in the presence of DNA-targeted therapies, SLFN11 is recruited to stressed replication forks where it blocks replication, leading to cell death (4). SLFN11 is being explored as a predictive biomarker for response to DNA-targeted therapies (5). Alternate Names FLJ34922; Schlafen family member 11; SLFN11; SLFN8/9; SLN11 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG