CST, 34865S, Phospho-p38 MAPK (Thr180/Tyr182) Matched Antibody Pair

Matched Antibody Pair for studying p38-alpha MAPK (Thr180/Tyr182) phosphate/p38-delta MAPK (Thr180/Tyr182) phosphate/p38-gamma MAPK (Thr183/Tyr185) phosphate/p38-beta MAPK (Thr180/Tyr182) phosphate in the research area. Product Usage Information Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Background p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Alternate Names CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; ERK-6; ERK3; ERK6; EXIP; Extracellular signal-regulated kinase 6; MAP kinase 11; MAP kinase 12; MAP kinase 13; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAP kinase p38 gamma; MAPK 11; MAPK 12; MAPK 13; MAPK 14; MAPK-13; MAPK11; MAPK12; MAPK13; MAPK14; MAX-interacting protein 2; MGC99536; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; Mitogen-activated protein kinase p38 delta; Mitogen-activated protein kinase p38 gamma; mitogen-activated protein kinase p38-2; MK11; MK12; MK13; MK14; MXI2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38-2; p38-alpha; P38A; p38ALPHA; p38alpha Exip; p38b; p38Beta; P38BETA2; P38D; p38delta; P38G; P38GAMMA; PRKM11; PRKM12; PRKM13; PRKM14; PRKM15; RK; SAPK-3; SAPK2; SAPK2A; SAPK2B; SAPK3; SAPK4; Stress-activated protein kinase 2; stress-activated protein kinase 2A; Stress-activated protein kinase 2b; Stress-activated protein kinase 3; Stress-activated protein kinase 4; stress-activated protein kinase-2; stress-activated protein kinase-2b Specification REACTIVITY: H M