CST, 34872T, TRF1 (F6G3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRF1/Pin2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRF1 (F6G3X) Rabbit Monoclonal Antibody (CST #34872) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRF1 (F6G3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRF1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect Pin2 isoform and not expected to detect TRF2 protein. By western blot and immunoprecipitation, this antibody detects an approximately 95 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala357 of human TRF1 protein. Background Telomeric Repeat-binding Factor 1 (TRF1) is one of six core proteins of the shelterin complex (TRF1, TRF2, Rap1, TIN2, POT1, and TPP1), which regulates telomere length and integrity. The shelterin complex localizes to telomeres and contributes to genomic stability by protecting telomeres from inappropriate processing by DNA repair pathways (1). Dysregulated expression of TRF1 promotes genomic instability and cancer progression in mice and humans. Both TRF1 and TRF2 are potential biomarkers and therapeutic targets in human cancer (2-4). Alternate Names FLJ41416; hTRF1-AS; NIMA-interacting protein 2; PIN2; t-TRF1; Telomeric protein Pin2/TRF1; telomeric repeat binding factor (NIMA-interacting) 1; telomeric repeat binding factor 1; telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF1; TRBF1; TRF; TRF1; TTAGGG repeat-binding factor 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG