CST, 3489T, MSK1 (C27B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MSK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MSK1 (C27B2) Rabbit Monoclonal Antibody (CST #3489) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MSK1 (C27B2) Rabbit Monoclonal Antibody detects endogenous levels of total MSK1 protein. This antibody detects a background band of unknown origin at 110 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn784 of human MSK1. Background MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well. Alternate Names 90 kDa ribosomal protein S6 kinase 5; KS6A5; MGC1911; MSK1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase A5; Ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RPS6KA5; RSK-like protein kinase; RSKL; S6K-alpha-5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG