CST, 34945T, ETEA/UBXD8 (D8H6D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBXD8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ETEA/UBXD8 (D8H6D) Rabbit Monoclonal Antibody (CST #34945) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ETEA/UBXD8 (D8H6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETEA/UBXD8 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly430 of human ETEA/UBXD8 protein. Background Ubiquitin regulatory X domain-containing protein 8 (UBXD8, also known as ETEA and FAF2) is a hairpin-anchored endoplasmic reticulum (ER) protein involved in ER associated degradation (ERAD). It influences this process by promoting translocation of misfolded proteins from the ER lumen to the cytoplasm for proteasome-mediated degradation (1). UBXD8 is a sensor for unsaturated fatty acids. In the absence of fatty acids UBXD8 binds to and targets INSIG1 for degradation, ultimately resulting in activation of SREBP-1. Under this condition, UBXD8 also inhibits triglyceride synthesis by blocking the conversion of diacylglycerols into triglycerides. Unsaturated fatty acids trigger UBXD8 polymerization and dissociation of UBXD8/INSIG1 complex, leading to feedback inhibition of SREBP-1 (2, 3). This induces UBXD8 to translocate from the ER to lipid droplets, where it binds to ATGL and inhibits its lipase activity (4, 5). The complex containing p97 and UBXD8 is reported to promote disassembly of the ribonucleoprotein complex to control mRNA stability (6). In addition, UBXD8 binds to and promotes degradation of neurofibromin (NF1), suggesting a role in regulating Ras activity (7). Alternate Names ETEA; expressed in T-cells and eosinophils in atopic dermatitis; FAF2; Fas associated factor family member 2; FAS-associated factor 2; KIAA0887; Protein ETEA; UBX domain containing 8; UBX domain protein 3B; UBX domain-containing protein 3B; UBX domain-containing protein 8; UBXD8; UBXN3B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG