CST, 3497T, PHF2 (D45A2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PHF2 (D45A2) Rabbit Monoclonal Antibody (CST #3497) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PHF2 (D45A2) Rabbit Monoclonal Antibody detects endogenous levels of total PHF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp585 human PHF2 protein. Background Plant homeodomain (PHD) finger protein 2 (PHF2) is a putative transcription factor. PHF2 contains a zinc finger-like PHD domain that is distinct from other classes of zinc finger motifs and is often found in proteins that influence chromatin structure (1). It also contains a Jumonji C (JmjC) domain, which may play a role in histone demethylation (2). The PHF2 gene is ubiquitously expressed in adult mouse tissues; however, the majority of PHF2 expression in the mouse embryo occurs in the neural tube and root ganglia (1). PHF2 mutations have been associated with both early- and late-onset breast carcinoma (3). Alternate Names CENP-35; centromere protein 35; GRC5; JHDM1E; jumonji C domain-containing histone demethylase 1E; KDM7C; KIAA0662; Lysine-specific demethylase PHF2; MGC176680; PHD finger protein 2; PHF2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG