CST, 35016S, Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated) (CST #35016) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:100 Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide. Background The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2a