CST, 3503S, Cortactin (H222) Antibody

Polyclonal Antibody for studying Cortactin. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cortactin (H222) Antibody (CST #3503) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cortactin (H222) Antibody detects endogenous levels of total cortactin protein. Species Reactivity: Human, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to centrally located residues of human cortactin. Antibodies are purified by protein A and peptide affinity chromatography. Background Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4). Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8). Alternate Names 1110020L01Rik; Amplaxin; cortactin; CTTN; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); epididymis secretory sperm binding protein; FLJ34459; Oncogene EMS1; Src substrate cortactin; SRC8 Specification REACTIVITY: H Mk B SENSITIVITY: Endogenous MW (kDa): 80-85 SOURCE: Rabbit