CST, 3504T, TBK1/NAK (D1B4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, TBK1/NAK (D1B4) Rabbit Monoclonal Antibody (CST #3504) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TBK1/NAK (D1B4) Rabbit Monoclonal Antibody detects endogenous levels of total TBK1/NAK protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser645 of human TBK1/NAK. Background TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3). Alternate Names FLJ11330; FTDALS4; IIAE8; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG