CST, 3505T, Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PABP1 (Arg455/Arg460) a-dimethyl. Validated for WB,IP,IHC,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody (CST #3505) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:25 Immunohistochemistry (Paraffin): 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 19351 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated. Background Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions, including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined. Alternate Names PAB1; PABP; PABP 1; PABP-1; PABP1; PABPC1; PABPC2; PABPL1; poly(A) binding protein cytoplasmic 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; Poly(A)-binding protein 1; Polyadenylate-binding protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 71 Source/Isotype: Rabbit IgG