CST, 35070S, SMARCD1/BAF60A (E7W9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMARCD1. Validated for WB,IP,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, SMARCD1/BAF60A (E7W9W) Rabbit Monoclonal Antibody (CST #35070) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq Specificity / Sensitivity SMARCD1/BAF60A (E7W9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMARCD1/BAF60A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly41 of human SMARCD1/BAF60A protein. Background The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8). SMARCD/BAF60 proteins are BAF family members that, along with SMARCC1/BAF155A, serve as an initial subcomplex critical for the formation of the intact BAF complex (9). These proteins serve as an interface between the BAF complex and many other key transcription factors, including glucocorticoid receptor and p53 (10,11). SMARCD1/BAF60A has been implicated in stem cell differentiation by controlling H3K27me3 distribution (12). Furthermore, SMARCD1/BAF60A has been shown to play a role in Notch signaling in glioblastomas (13). Alternate Names 60 kDa BRG-1/Brm-associated factor subunit A; BAF60A; BRG1-associated factor 60A; chromatin remodeling complex BAF60A subunit; CRACD1; CSS11; mammalian chromatin remodeling complex BRG1-associated factor 60A; Rsc6p; SMARCD1; SMRD1; SWI/SNF complex 60 kDa subunit; SWI/SNF complex 60 kDa subunit A; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; Swp73-like protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG