CST, 3507S, Sav1 Antibody

Polyclonal Antibody for studying SAV1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Sav1 Antibody (CST #3507) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Sav1 Antibody detects endogenous level of total Sav1 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human Sav1. Antibodies are purified by protein A and peptide affinity chromatography. Background Salvador homolog (SAV1), originally named WW45, was first identified as a 45 kDa protein containing a pair of WW domains and a coiled-coil region (1). SAV1 was subsequently shown to function as a scaffold protein, in a protein complex that includes the kinases MST2 and LATS1, and the transcriptional co-activator YAP (2). This protein complex comprises the core components of the Hippo signaling pathway, which regulates important cellular functions, including contact inhibition and apoptosis, that function to regulate tissue growth and organ size (3,4). A genetic screen in identified a role for SAV1 in cell cycle regulation and apoptosis (5), while embryonic mice lacking Sav1 displayed hyperplastic growth and epithelial differentiation effects (6). These findings, together with the observation that SAV1 is mutated a number of human cancer cell lines, suggest that SAV1 functions as a tumor suppressor in the Hippo signaling pathway (5, 7). Alternate Names 1700040G09Rik; 45 kDa WW domain protein; hWW45; Protein salvador homolog 1; salvador family WW domain containing protein 1; salvador homolog 1; salvador homolog 1 (Drosophila); SAV; SAV1; WW domain-containing; WW domain-containing adaptor 45; WW45; WWP4 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit